Additional Selective and Differential Cell Culture Media
NEOGEN Lactobacilli MRS Broth, 10 kg
Lactobacilli MRS Broth is used for the cultivation of lactobacilli and is not intended for use in the diagnosis of disease or other conditions in humans.Lactobacilli MRS broth was originally developed in 1960 by de Man, Rogosa & Sharpe, the medium can be used for confirmatory tests on organisms isolated on MRS Agar. The medium can also be used for enumeration by the Miles and Misra technique.Nutrition is provided by a mixture of carefully selected peptones, glucose, beef and yeast extracts while Tween 80, magnesium and manganese sulfates act as growth stimulants. Selectivity against streptococci & molds is provided by ammonium citrate and sodium acetate.For more information visit https://www.neogen.com/solutions/Microbiology/lactobacilli-mrs-broth
NEOGEN DRBC Agar (ISO), 500 g
Dichloran Rose Bengal Chloramphenicol Agar is for the enumeration of yeasts and is not intended for use in the diagnosis of disease or other conditions in humans.Developed with reference to ISO 21527-1:2008 for the enumeration of yeasts and molds in food and animal products, this medium is tested to the performance requirements of the standard. Used for the enumeration of viable yeasts and molds in products with a water activity of greater than 0.95 such as eggs, meat, some dairy products, fresh pastes, fruit and vegetables, DRBC Agar is designed to suppress the colonial growth of ‘spreader’ molds and in doing so allow easier performance of the colony count technique on yeasts and molds.For more information visit https://www.neogen.com/solutions/Microbiology/drbc-agar-iso
NEOGEN Modified Buffered Peptone Water with Pyruvate, 500 g
Modified Buffered Peptone Water with Pyruvate (mBPWp) is used for the isolation of Enterohemorrhagic E. coli (EHEC) in a laboratory setting. Modified Buffered Peptone Water with Pyruvate (mBPWp) is not intended for use in the diagnosis of disease or other conditions in humans. Enterohemorrhagic E. coli (EHEC) are recognized as the primary cause of hemorrhagic colitis (HC) or bloody diarrhea. This infection can also lead to hemolytic uremic syndrome (HUS). Enterohemorrhagic infections are usually food or water borne and have been implicated in undercooked ground beef, raw milk, lunchmeat, water, unpasteurized apple cider, sprouts and vegetables. Modified Buffered Peptone Water with Pyruvate is recommended in the sample preparation for the screening method following FDA/BAM Enterohemorrhagic E. coli (EHEC) Protocol.For more information visit https://www.neogen.com/solutions/Microbiology/modified-buffered-peptone-water-with-pyruvate
NEOGEN Maximum Recovery Diluent (Tryptone Salt Broth), 500 g
Maximum Recovery Diluent (Tryptone Salt Broth) is used as an isotonic diluent for maximum recovery of microorganisms and is not intended for use in the diagnosis of disease or other conditions in humans. An osmotically controlled solution which is an alternative to, and a replacement for, 1/4 strength Ringer’s Tablets (NCM0191K). The presence of a low level of peptone lessens the physiological shock normally experienced by bacterial cells when they are introduced to a diluent such as Ringer’s Solution. The level of peptone is such that multiplication of the organisms is not possible in the time in which the sample will be present in the diluent (1-2 hours). This formula is recommended by ISO 6887-1:2017 For more information visit https://www.neogen.com/solutions/Microbiology/maximum-recovery-diluent-tryptone-salt-broth
NEOGEN Bismuth Sulphite Agar, 500 g
Bismuth Sulfite Agar is used for the selective isolation of Salmonella spp. In a laboratory setting. Bismuth Sulfite Agar is not intended for use in the diagnosis of disease or other conditions in humans and conforms to FDA/BAM formulation. Salmonellosis can result from consumption of raw, undercooked, or improperly processed foods contaminated with Salmonella. U. S. federal guidelines require various poultry products to be routinely monitored before distribution for human consumption. Bismuth Sulfite Agar is a modification of Wilson and Blair formula. The typhoid organism grows luxuriantly on the medium, forming characteristic black colonies. Gram-positive bacteria and coliforms are inhibited on Bismuth Sulfite Agar. The inhibitory action of Bismuth Sulfite Agar permits the use of a large inoculum, increasing the possibility of recovering pathogens that may be present in small numbers.For more information visit https://www.neogen.com/solutions/Microbiology/bismuth-sulfite-agar
NEOGEN Bismuth Sulphite Agar, 10 kg
Bismuth Sulfite Agar is used for the selective isolation of Salmonella spp. In a laboratory setting. Bismuth Sulfite Agar is not intended for use in the diagnosis of disease or other conditions in humans and conforms to FDA/BAM formulation. Salmonellosis can result from consumption of raw, undercooked, or improperly processed foods contaminated with Salmonella. U. S. federal guidelines require various poultry products to be routinely monitored before distribution for human consumption. Bismuth Sulfite Agar is a modification of Wilson and Blair formula. The typhoid organism grows luxuriantly on the medium, forming characteristic black colonies. Gram-positive bacteria and coliforms are inhibited on Bismuth Sulfite Agar. The inhibitory action of Bismuth Sulfite Agar permits the use of a large inoculum, increasing the possibility of recovering pathogens that may be present in small numbers.For more information visit https://www.neogen.com/solutions/Microbiology/bismuth-sulfite-agar
NEOGEN LB Broth (Miller), 500 g
LB Broth (Miller) is used in molecular genetic studies in a laboratory setting. LB Broth, Miller is not intended for use in the diagnosis of disease or other conditions in humans. LB Broth (Miller) is based on the formula described by Miller. This medium is used for the growth and maintenance of Escherichia coli strains used in molecular microbiology procedures. LB Broth, Miller is a nutritionally rich medium designed for growth of pure cultures of recombinant strains. E. coli is grown to late log phase in LB Medium. Some plasmid vectors replicate to high copy numbers without selective amplification. Some vectors do not replicate so freely and need to be selectively amplified. Chloramphenicol can be added to inhibit host synthesis and prevent replication of the bacterial chromosome. This broth contains a high level of sodium chloride to aid the maintenance of plasmids.For more information visit https://www.neogen.com/solutions/Microbiology/lb-broth-miller
NEOGEN Violet Red Bile Agar (VRBA) (ISO), 5 kg
A medium for the enumeration of coliform organisms in food and dairy products according to ISO 4832:2006 and is not intended for use in the diagnosis of disease or other conditions in humans.The selectivity of the medium is due to the presence of bile salts and crystal violet. Lactose fermenters produce red/purple colonies often surrounded by a halo of the same color. Non-lactose fermenters produce pale colonies. Selectivity can be increased by incubation at 42-44°C.Formula:Yeast Extract - 3.0 g/LEnzymatic Digest of Animal Tissue - 7.0 g/LBile Salts - 1.5 g/LLactose - 10.0 g/LNeutral Red - 0.03 g/LCrystal Violet - 0.002 g/LAgar- 12.0 g/LFor more information visit https://www.neogen.com/solutions/Microbiology/violet-red-bile-lactose-agar-iso
NEOGEN Tetrathionate Broth Base, 10 kg
Tetrathionate Broth Base is used with iodine for the recovery of Salmonella spp. in a laboratory setting. Tetrathionate Broth Base is not intended for use in the diagnosis of disease or other conditions in humans. Tetrathionate Broth Base is used as a selective enrichment for the cultivation of Salmonella spp. Salmonella organisms may be injured in food-processing procedures, which include exposure to low temperatures, sub-marginal heat, drying, radiation, preservative, and sanitizers. Mueller demonstrated the effectiveness of Tetrathionate Broth for enriching typhoid and paratyphoid bacilli while inhibiting coliform organisms. Using modified Mueller’s broth, Kauffmann increased the number of positive isolates. Tetrathionate Broth was used in studies for the poultry industry and in a collaborative study for rapid screening of Salmonella in food.For more information visit https://www.neogen.com/solutions/Microbiology/tetrathionate-broth-base
NEOGEN Tetrathionate Broth Base, 500 g
Tetrathionate Broth Base is used with iodine for the recovery of Salmonella spp. in a laboratory setting. Tetrathionate Broth Base is not intended for use in the diagnosis of disease or other conditions in humans. Tetrathionate Broth Base is used as a selective enrichment for the cultivation of Salmonella spp. Salmonella organisms may be injured in food-processing procedures, which include exposure to low temperatures, sub-marginal heat, drying, radiation, preservative, and sanitizers. Mueller demonstrated the effectiveness of Tetrathionate Broth for enriching typhoid and paratyphoid bacilli while inhibiting coliform organisms. Using modified Mueller’s broth, Kauffmann increased the number of positive isolates. Tetrathionate Broth was used in studies for the poultry industry and in a collaborative study for rapid screening of Salmonella in food.For more information visit https://www.neogen.com/solutions/Microbiology/tetrathionate-broth-base
NEOGEN Brucella Agar, 500 g
Brucella Agar is used for the cultivation of Brucella spp. and other fastidious microorganisms in a laboratory setting. Brucella Agar is not intended for use in the diagnosis of disease or other conditions in humans.Brucella Agar is prepared according to the APHA formula for Albimi Broth. Brucella Agar is a general-purpose medium for the cultivation of Brucella spp. and fastidious microorganisms including Streptococcus pneumoniae, Streptococcus viridans, and Neisseria meningitidis. With the addition of blood, Brucella Agar is used to determine bacterial hemolytic reactions. Brucella Agar can also be used as a base for the isolation of Campylobacter spp.For more information visit https://www.neogen.com/solutions/Microbiology/brucella-agar
NEOGEN Malt Extract Agar, 5 kg
Malt Extract Agar is used for the cultivation of fungi and is not intended for use in the diagnosis of disease or other conditions in humans. An acidic medium which will support the growth of most yeasts and molds whilst inhibiting most bacteria. It was first described by Thom and Church in 1926 in a study of Aspergillus spp. claiming the high carbohydrate content ensured rapid growth. Selectivity can be increased by further lowering the pH with the addition, after sterilization, of Lactic Acid (X037). It should be noted that excess heating of this medium together with its low pH can easily result in hydrolysis of the agar gel producing soft plates. Formula:Malt Extract - 30.0 g/LPeptone - 5.0 g/LAgar - 15.0 g/LFor more information visit https://www.neogen.com/solutions/Microbiology/malt-extract-agar
NEOGEN Peptone Water, 5 kg
Peptone Water is used for the cultivation of non-fastidious microorganisms, indole testing, and as a basal medium for carbohydrate fermentation studies. Peptone Water is not intended for use in the diagnosis of disease or other conditions in humans. A general purpose growth medium that can be used as a base for carbohydrate fermentation studies. The medium has a high level of tryptone making it suitable for use in the indole test.For more information visit https://www.neogen.com/solutions/Microbiology/peptone-water
NEOGEN Sabouraud Dextrose Agar with Lecithin & Tween, 500 g
Sabouraud Dextrose Agar (with Lecithin & Tween) is used for the isolation of fungi from surfaces sanitized with quaternary ammonium compounds in a laboratory setting. Sabouraud Dextrose Agar (with Lecithin & Tween) is not intended for use in the diagnosis of disease or other conditions in humans. Sabouraud Dextrose Agar (SDA) is a modification of Dextrose Agar described by Sabouraud. SDA is used for cultivating pathogenic and commensal fungi and yeasts. The high dextrose concentration and acidic pH of the formula permits selectivity of fungi. The addition of Lecithin and Tween to Sabouraud Dextrose Agar is used to neutralize antiseptics and disinfectants for environmental monitoring and other applications. Complete neutralization of disinfectants is important. Disinfectant carryover can cause false no-growth test results.For more information visit https://www.neogen.com/solutions/Microbiology/sabouraud-dextrose-agar-with-lecithin-and-tween
NEOGEN Peptone Water, 500 g
Peptone Water is used for the cultivation of non-fastidious microorganisms, indole testing, and as a basal medium for carbohydrate fermentation studies. Peptone Water is not intended for use in the diagnosis of disease or other conditions in humans. A general purpose growth medium that can be used as a base for carbohydrate fermentation studies. The medium has a high level of tryptone making it suitable for use in the indole test.For more information visit https://www.neogen.com/solutions/Microbiology/peptone-water